Changjun You, Pengcheng Wang, Stephanie L. Nay, Jianshuang Wang, Xiaoxia Dai, Timothy R. O’Connor, and Yinsheng Wang
ACS Chem. Biol., Articles ASAP (As Soon As Publishable)
Publication Date (Web): March 1, 2016 (Articles)
DOI: 10.1021/acschembio.6b00085
Environmental and endogenous genotoxic agents can result in a variety of alkylated and carboxymethylated DNA lesions, including N3-ethylthymidine (N3-EtdT), O2-EtdT, and O4-EtdT as well as N3-carboxymethylthymidine (N3-CMdT) and O4-CMdT. By using ...
Tao Ran, Hengzhi Li, Yong Liu, Chuanshe Zhou, Shaoxun Tang, Xuefeng Han, Min Wang, Zhixiong He, Jinghe Kang, Qiongxian Yan, Zhiliang Tan, and Karen A. Beauchemin
J. Agric. Food Chem., 2016, 64 (11), pp 2333–2341
Publication Date (Web): February 25, 2016 (Article)
DOI: 10.1021/acs.jafc.5b06131
G-protein-coupled receptor 120 (GPR120) is reported as a long-chain fatty acid (LCFA) receptor that elicits free fatty acid (FFA) regulation on metabolism homeostasis. The study aimed to clone the gpr120 gene of goats (g-GPR120) and subsequently ...
Kyle J. Anderson, Katie L. Vermillion, Pratik Jagtap, James E. Johnson, Timothy J. Griffin, and Matthew T. Andrews
J. Proteome Res., 2016, 15 (4), pp 1253–1261
Publication Date (Web): February 23, 2016 (Article)
DOI: 10.1021/acs.jproteome.5b01138
Mammalian hibernation is a strategy employed by many species to survive fluctuations in resource availability and environmental conditions. Hibernating mammals endure conditions of dramatically depressed heart rate, body temperature, and oxygen ...
Daniel M. Schenck and Jennifer Fiegel
ACS Appl. Mater. Interfaces, 2016, 8 (9), pp 5917–5928
Publication Date (Web): February 19, 2016 (Research Article)
DOI: 10.1021/acsami.6b00294
Lung surfactant has been observed at all surfaces of the airway lining fluids and is an important contributor to normal lung function. In the conducting airways, the surfactant film lies atop a viscoelastic mucus gel. In this work, we report on the ...
Takahiro Masuda and Marco Prinz
ACS Chem. Neurosci., Articles ASAP (As Soon As Publishable)
Publication Date (Web): February 4, 2016 (Review)
DOI: 10.1021/acschemneuro.5b00317
Microglia are the resident monocytic cells in the central nervous system (CNS), where they constitute the complex tissue structure together with a diverse set of cell-types, including neurons, glial cells, and vasculature. Different from other cells, ...
Adam Facey, Isaac Pinar, Jane F. Arthur, Jianlin Qiao, Jing Jing, Belden Mado, Josie Carberry, Robert K. Andrews, and Elizabeth E. Gardiner
Biochemistry, 2016, 55 (8), pp 1187–1194
Publication Date (Web): February 3, 2016 (Article)
DOI: 10.1021/acs.biochem.5b01102
The primary platelet collagen receptor, glycoprotein VI (GPVI), plays an important role in platelet activation and thrombosis. The ectodomain of human GPVI (sGPVI) is proteolytically shed from human platelets by a-disintegrin-and-metalloproteinase 10 (...
Varvara Gribova, Isabelle Pignot-Paintrand, Laure Fourel, Rachel Auzely-Velty, Corinne Albigès-Rizo, Cécile Gauthier-Rouvière, and Catherine Picart
ACS Biomater. Sci. Eng., 2016, 2 (3), pp 415–425
Publication Date (Web): January 28, 2016 (Article)
DOI: 10.1021/acsbiomaterials.5b00557
Controlling the different steps of cell differentiation in vitro using bioactive surfaces may be useful in view of future cell therapies. Substrates presenting peptides, which are minimal fragments of extracellular matrix (ECM) proteins may be used for ...
Fumiaki Ogawa, Laura C. Murphy, Elise L. V. Malavasi, Shane T. O’Sullivan, Helen S. Torrance, David J. Porteous, and J. Kirsty Millar
ACS Chem. Neurosci., Articles ASAP (As Soon As Publishable)
Publication Date (Web): January 27, 2016 (Research Article)
DOI: 10.1021/acschemneuro.5b00255
Mitochondria are essential for neuronal function, providing the energy required to power neurotransmission, and fulfilling many important additional roles. In neurons, mitochondria must be efficiently transported to sites, including synapses, where their ...
Andy Tay, Anja Kunze, Coleman Murray, and Dino Di Carlo
ACS Nano, 2016, 10 (2), pp 2331–2341
Publication Date (Web): January 25, 2016 (Article)
DOI: 10.1021/acsnano.5b07118
Nanomagnetic force stimulation with ferromagnetic nanoparticles was found to trigger calcium influx in cortical neural networks without observable cytotoxicity. Stimulated neural networks showed an average of 20% increment in calcium fluorescence signals ...
Laxmikanth Kollipara, Stephan Buchkremer, Joachim Weis, Eva Brauers, Mareike Hoss, Stephan Rütten, Pablo Caviedes, René P. Zahedi, and Andreas Roos
J. Proteome Res., 2016, 15 (3), pp 945–955
Publication Date (Web): January 19, 2016 (Article)
DOI: 10.1021/acs.jproteome.5b00972
Studying (neuro)muscular disorders is a major topic in biomedicine with a demand for suitable model systems. Continuous cell culture (in vitro) systems have several technical advantages over in vivo systems and became widely used tools for discovering ...
Adam W. Smith, Hector H. Huang, Nicholas F. Endres, Christopher Rhodes, and Jay T. Groves
J. Phys. Chem. B, 2016, 120 (5), pp 867–876
Publication Date (Web): January 15, 2016 (Article)
DOI: 10.1021/acs.jpcb.5b08887
The spatial organization of lipid-anchored proteins in the plasma membrane directly influences cell signaling, but measuring such organization in situ is experimentally challenging. The canonical oncogene, c-Src, is a lipid anchored protein that plays a ...
Jack D. Wang, El-Sayed Khafagy, Khalil Khanafer, Shuichi Takayama, and Mohamed E. H. ElSayed
Mol. Pharmaceutics, 2016, 13 (3), pp 895–906
Publication Date (Web): January 10, 2016 (Article)
DOI: 10.1021/acs.molpharmaceut.5b00805
The endothelial cells lining the capillaries supplying the brain with oxygen and nutrients form a formidable barrier known as the blood–brain barrier (BBB), which exhibits selective permeability to small drug molecules and virtually impermeable to ...
Cesare Castellini, Alessandro Dal Bosco, Simona Mattioli, Magdalena Davidescu, Lanfranco Corazzi, Lara Macchioni, Simona Rimoldi, and Genciana Terova
J. Agric. Food Chem., 2016, 64 (4), pp 792–800
Publication Date (Web): January 8, 2016 (Article)
DOI: 10.1021/acs.jafc.5b05425
In the present paper liver fatty acid Δ6 desaturation (fads2) activity was analyzed in two rabbit strains with slow- (S, 27.5 g/day) or fast-growing (F, 48.5 g/day) rate. The fatty acid profile of the liver showed a different PUFA profile in the two ...
Jie-Yi Ma, Ka-Lok Wong, Zhen-Yan Xu, Ka-Yee Au, Nga-Lam Lee, Chang Su, Wei-Wei Su, Pei-Bo Li, and Pang-Chui Shaw
J. Nat. Prod., 2016, 79 (1), pp 204–212
Publication Date (Web): January 7, 2016 (Article)
DOI: 10.1021/acs.jnatprod.5b00854
N16 is a protein from the nacreous layer of Pinctada fucata, a pearl oyster. It has been found to promote biomineralization, and we hypothesized that it also plays a role in bone metabolism. The cDNA of N16 was cloned and expressed in Escherichia coli to ...
Bryan E. Thacker, Emylie Seamen, Roger Lawrence, Matthew W. Parker, Yongmei Xu, Jian Liu, Craig W. Vander Kooi, and Jeffrey D. Esko
ACS Chem. Biol., 2016, 11 (4), pp 971–980
Publication Date (Web): January 5, 2016 (Articles)
DOI: 10.1021/acschembio.5b00897
Binding of proteins to heparan sulfate is driven predominantly by electrostatic interactions between positively charged amino acid residues in the protein and negatively charged sulfate groups located at various positions along the polysaccharide chain. ...
Marc Simon, Maxim Dokukin, Vivekanand Kalaparthi, Elise Spedden, Igor Sokolov, and Cristian Staii
Langmuir, 2016, 32 (4), pp 1111–1119
Publication Date (Web): January 4, 2016 (Article)
DOI: 10.1021/acs.langmuir.5b04317
When studying the mechanical properties of cells by an indentation technique, it is important to take into account the nontrivial pericellular interface (or pericellular “brush”) which includes a pericellular coating and corrugation of the pericellular ...
Li Jiang, Zhenglong Sun, Xiaofei Chen, Jing Li, Yue Xu, Yan Zu, Jiliang Hu, Dong Han, and Chun Yang
ACS Nano, 2016, 10 (1), pp 207–217
Publication Date (Web): December 23, 2015 (Article)
DOI: 10.1021/acsnano.5b03157
The question of how cells sense substrate mechanical cues has gained increasing attention among biologists. By introducing contour-based data analysis to single-cell force spectroscopy, we identified a loading-rate threshold for the integrin α2β1–DGEA ...
Karina H. Nakayama, Vinay N. Surya, Monica Gole, Travis W. Walker, Weiguang Yang, Edwina S. Lai, Maggie A. Ostrowski, Gerald G. Fuller, Alexander R. Dunn, and Ngan F. Huang
Nano Lett., 2016, 16 (1), pp 410–419
Publication Date (Web): December 16, 2015 (Letter)
DOI: 10.1021/acs.nanolett.5b04028
The role of nanotopographical extracellular matrix (ECM) cues in vascular endothelial cell (EC) organization and function is not well-understood, despite the composition of nano- to microscale fibrillar ECMs within blood vessels. Instead, the predominant ...
Kornelia Galior, Yang Liu, Kevin Yehl, Skanda Vivek, and Khalid Salaita
Nano Lett., 2016, 16 (1), pp 341–348
Publication Date (Web): November 24, 2015 (Letter)
DOI: 10.1021/acs.nanolett.5b03888
Mechanical forces transmitted through integrin transmembrane receptors play important roles in a variety of cellular processes ranging from cell development to tumorigenesis. Despite the importance of mechanics in integrin function, the magnitude of ...
Yun Wang, Iqbal Javed, Yahui Liu, Song Lu, Guang Peng, Yongqian Zhang, Hong Qing, and Yulin Deng
J. Proteome Res., 2016, 15 (1), pp 29–37
Publication Date (Web): November 2, 2015 (Article)
DOI: 10.1021/acs.jproteome.5b00777
Mitochondria are not only the main source of energy in cells but also produce reactive oxygen species (ROS), which result in oxidative stress when in space. This oxidative stress is responsible for energy imbalances and cellular damage. In this study, a ...
