White Cube Bermondsey opened in October 2011 and is the largest of all the gallery's sites, incorporating more than 5440m² (58,000 sq ft) of interior space. The building, which dates from the 1970s, was renovated and designed by London and Berlin-based architects Casper Mueller Kneer and includes three major exhibition spaces as well as private viewing rooms, office space, a warehouse, an auditorium and a bookshop. The 'South Galleries' provide the principal display area for White Cube's expanding programme of exhibitions and three smaller galleries, known collectively as the 'North Galleries', are used for an innovative series of shows. In addition, at the centre of the building, a top-lit, 81m² gallery entitled '9 x 9 x 9', is used for special projects or for the display of a single artwork or installation. Since its inception, the building has hosted a variety of important exhibitions such as the first UK showing of work by American artist Theaster Gates, a comprehensive retrospective of prints by Chuck Close and the largest presentation of Anselm Kiefer work's ever staged in London. To accompany these exhibitions, an education programme and an ongoing series of artists films, feature films and lectures takes place in the purpose-built 60 seat auditorium.

White Cube Mason's Yard opened in September 2006 in a courtyard off Duke Street St James', nearby to the original White Cube space. The building, constructed on the site of an electricity sub-station and designed by London based MRJ Rundell & Associates, is the first free-standing structure to be built in the historic St James area for more than 30 years. The building houses two major galleries that provide a total of 1110m² (11,900 sq ft) of exhibition space, comprising of a ground floor gallery on street level and a double-height, naturally-lit basement gallery. Used for White Cube's expanding programme of exhibitions, White Cube Mason's Yard has hosted a wide range of exhibitions by international artists including Andreas Gursky, Georg Baselitz, Jeff Wall, Anselm Kiefer, Robert Irwin and Miroslaw Balka. The gallery opened with an inaugural exhibition by Mexican artist Gabriel Orozco.

The first of White Cube's galleries to be located outside of the UK, White Cube Hong Kong is situated at 50 Connaught Road, in the heart of Hong Kong's Central district. Since its inception in March 2012, the gallery has hosted a varied programme of exhibitions including Gilbert & George, Anselm Kiefer, Damien Hirst and Cerith Wyn Evans. The gallery provides an interior exhibition space of 550 m2 (6000 sq ft) which is set over two floors and has a ceiling height of over 4.5 metres. The building was designed by London based architects Maybank and Matthews.

'Inside the White Cube' is a programme of exhibitions profiling work by artists who have not previously shown at the gallery. The series provides a stimulating platform for exploring new developments in international contemporary art, across a range of practices and media. Based at White Cube Bermondsey, the exhibitions are adaptable in content and, on occasion, migratory in location: from the north galleries, south galleries and auditorium to courtyard and external sites. 

White Cube São Paulo opened in 2012, becoming the second venue for White Cube outside the UK. Following on from the success of a one-off, special project – Facts and Systems by Antony Gormley – White Cube committed to a programme of major exhibitions for three years in Brazil. Housed in a converted warehouse that was designed by London based Maybank and Matthews Architects with São Paulo based Estudio Gru, the gallery provided 298 sq m / 3207 sq ft of exhibition space in the centre of the city.

White Cube Hoxton Square opened in April 2000 in the East End of London, as the second, larger gallery space to the original White Cube. Situated on Hoxton Square in Old Street, the light industrial building from the 1920s was re-designed and re-purposed by London based architects MRJ Rundell and Associates. With 890m² (9,500 sq ft) of gallery space spread over two floors, the building housed a series of solo and group exhibitions by leading international artists. After a consistently challenging programme that lasted for 12 years, the gallery closed in December 2012.

White Cube was set up by Jay Jopling in 1993 as a project room for contemporary art. Although it was one of the smallest exhibition spaces in Europe (measuring just 2.92 x 4.64 x 4.42 m) it was arguably one of most influential commercial galleries of the past decade. Situated on Duke Street, St James’s, one of London’s most traditional art dealing streets, the gallery was a perfect white cube, a room within a room, designed by the architect Claudio Silvestrin.

The central concern when establishing the programme was to create an intimate space in which an artist could present a single important work of art or a coherent body of work within a focused environment, an idea that, in some way, stemmed from the memorable experience of Walter de Maria’s Earth Room in New York. The programme was singular among commercial galleries in that an artist was invited to exhibit only once. Since its inception, the gallery mounted exhibitions of work by many leading international and British artists including Franz Ackermann, Miroslaw Balka, Chuck Close, Tracey Emin, Katharina Fritsch, Mona Hatoum, Damien Hirst, Gary Hume, Ellsworth Kelly, Julie Mehretu, Doris Salcedo, Hiroshi Sugimoto, Luc Tuymans and Jeff Wall. White Cube, Duke Street closed in 2002.