A protocol for in vivo detection of reactive oxygen species

Authors: Edward Owusu-Ansah, Amir Yavari, Utpal Banerjee

Lab groups: Banerjee Lab (University of California)

• Associated Publications:

  • Distinct mitochondrial retrograde signals control the G1-S cell cycle checkpoint

2’,7’-dichlorofluorescein (H2DCF) and Dihydroethidium (DHE), have been used extensively in tissue culture experiments to evaluate reactive oxygen species (ROS) production. However, i...

Neural Stem Cell Culture: Neurosphere generation, microscopical analysis and cryopreservation

Authors: Laura Pacey KK, Shelley Stead, Jacqueline Gleave A, Kasia Tomczyk, Laurie Doering C

Lab groups: Department of Pathology and Molecular Medicine, McMaster University

Essential technical tips for STAP cell conversion culture from somatic cells

Authors: Haruko Obokata, Yoshiki Sasai, Hitoshi Niwa

Lab groups: STAP Group RIKEN CDB

• Associated Publications:

  • Stimulus-triggered fate conversion of somatic cells into pluripotency
  • Bidirectional developmental potential in reprogrammed cells with acquired pluripotency

THE AUTHORS ARE RETRACTING THIS PROTOCOL EXCHANGE PROTOCOL BECAUSE THE PAPERS UPON WHICH IT IS BASED HAVE BEEN RETRACTED. Stimulus-triggered acquisition of pl...

Anisotropic Mobilities in Organic Semiconductors

Authors: Keli Han, Jindou Huang, Shuo Chai, Shuhao Wen, Weiqiao Deng

Lab groups: Han's Lab, Dalian Institute of Chemical Physics

• Associated Publications:

  • Ultra-low resistance at TTF–TCNQ organic interfaces
  • First-Principles Investigation of Anistropic Hole Mobilities in Organic Semiconductors
  • Revealing quantitative structure–activity relationships of transport properties in acene and acene derivative organic materials
  • Simulation of Hole Mobility in α-Oligofuran Crystals

As one of the most important physical properties of the organic material, the anisotropic charge-carrier mobility directly determines device performance to a large extent. In this protocol, we main...

Production of neuron-preferential lentiviral vectors

Authors: Takashi Torashima, Chiho Koyama, Haruhiro Higashida, Hirokazu Hirai

Lab groups: H. Higashida Lab (Kanazawa Univ)

• Associated Publications:

  • CD38 is critical for social behaviour by regulating oxytocin secretion

Adenoviral vectors widely used to transfer foreign genes into neuronal cells possess tropism for glial cells 1, 2 and are toxic to infected cells. Alternatively, the use of lentiviral vectors for t...

Enhancing graphene/CNT based electrochemical detection using magneto-nanobioprobes

Authors: Priyanka Sharma, V Bhalla, E Senthil Prasad, V Dravid, G Shekhawat, C. Raman Suri

Lab groups: Nanobiotech and Biosensor Lab, IMTECH, Chandigarh

• Associated Publications:

  • Enhancing electrochemical detection on graphene oxide-CNT nanostructured electrodes using magneto-nanobioprobes

This protocol describes an optimized signal amplification strategy to develop an ultra-sensitive magneto-electrochemical biosensing platform. The new protocol combines the advantages of carbon nano...

Fabrication of Nanoelectrospray Emitters for LC-MS

Authors: Paolo Cifani, Avantika Dhabaria, Alex Kentsis

Lab groups: Kentsis Research Group

Ionization efficiency and consequently the sensitivity of mass spectrometric analyses strongly depend on the geometry of nanoelectrospray emitter used, particularly on the terminal inner diameter (...

Ribose-seq: ribonucleotides in DNA to Illumina library

Authors: Kyung Duk Koh, Jay Hesselberth, Francesca Storici

Lab groups: Storici Lab

• Associated Publications:

  • Ribose-seq: global mapping of ribonucleotides embedded in genomic DNA

Ribonucleotides are now widely considered as the most frequently incorporated non-canonical nucleotides in genomic DNA. Here, we describe an approach, ribose-seq, to prepare DNA libraries for next-...

Epiblast grafting and in vitro embryo culture

Authors: Jun Wu, Daiji Okamura, Juan Carlos Izpisua Belmonte

Lab groups: Belmonte Lab (Salk)

• Associated Publications:

  • An alternative pluripotent state confers interspecies chimaeric competency

This protocol describes a method to graft conventional primed pluripotent stem cells (PSCs) and region-selective PSCs to different locations of post-implantation mouse epiblast followed by in vitro...

A protocol for in vivo detection of reactive oxygen species

Authors: Edward Owusu-Ansah, Amir Yavari, Utpal Banerjee

Lab groups: Banerjee Lab (University of California)

• Associated Publications:

  • Distinct mitochondrial retrograde signals control the G1-S cell cycle checkpoint

2’,7’-dichlorofluorescein (H2DCF) and Dihydroethidium (DHE), have been used extensively in tissue culture experiments to evaluate reactive oxygen species (ROS) production. However, i...

Neural Stem Cell Culture: Neurosphere generation, microscopical analysis and cryopreservation

Authors: Laura Pacey KK, Shelley Stead, Jacqueline Gleave A, Kasia Tomczyk, Laurie Doering C

Lab groups: Department of Pathology and Molecular Medicine, McMaster University

Essential technical tips for STAP cell conversion culture from somatic cells

Authors: Haruko Obokata, Yoshiki Sasai, Hitoshi Niwa

Lab groups: STAP Group RIKEN CDB

• Associated Publications:

  • Stimulus-triggered fate conversion of somatic cells into pluripotency
  • Bidirectional developmental potential in reprogrammed cells with acquired pluripotency

THE AUTHORS ARE RETRACTING THIS PROTOCOL EXCHANGE PROTOCOL BECAUSE THE PAPERS UPON WHICH IT IS BASED HAVE BEEN RETRACTED. Stimulus-triggered acquisition of pl...